Journal: Veterinary Sciences

Article Title: Characterization of Two Porcine Parainfluenza Virus 1 Isolates and Human Parainfluenza Virus 1 Infection in Weaned Nursery Pigs

doi: 10.3390/vetsci10010018

Figure Lengend Snippet: Experimental design comparing challenge with Human parainfluenza virus 1 (ATCC ® VR-94™), and Porcine parainfluenza virus 1 isolates USA/MN25890NS/2016 and USA/IA84915LG/2017 in conventional nursery pigs. NA: Not applicable.

Article Snippet: HPIV1 , Strain C35 ATCC ® VR-94 TM , JQ901971 , 8 , 5 , 3.

Techniques: Virus

Journal: Journal of Virological Methods

Article Title: A novel multiplex PCR for virus detection by melting curve analysis

doi: 10.1016/j.jviromet.2018.09.010

Figure Lengend Snippet: Primers and probes used for the multiplex PCR.

Article Snippet: PIV1 (ATCC-VR94) and PIV2 (ATCC-VR92) were obtained from the Shanghai Medical College of Fudan University.

Techniques: Multiplex Assay, Sequencing, Concentration Assay

Journal: Journal of Virological Methods

Article Title: A novel multiplex PCR for virus detection by melting curve analysis

doi: 10.1016/j.jviromet.2018.09.010

Figure Lengend Snippet: Performance of the multiplex PCR for clinical samples compared with the direct fluorescent antibody testing.

Article Snippet: PIV1 (ATCC-VR94) and PIV2 (ATCC-VR92) were obtained from the Shanghai Medical College of Fudan University.

Techniques: Multiplex Assay

Journal: International Journal of Oncology

Article Title: Salicylic acid metabolites and derivatives inhibit CDK activity: Novel insights into aspirin's chemopreventive effects against colorectal cancer

doi: 10.3892/ijo.2017.4167

Figure Lengend Snippet: Effect of aspirin, salicylic acid, 2,3-DHBA, 2,6-DHBA and 2,4,6-THBA (at 0.5 mM) on CDK2, 4 and 6 enzyme activity. (A) Upper panel represents in vitro kinase assays showing the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA on CDK2 activity. Lower panel shows coomassie stained H1 histones. (B) Quantification of the blot in (A). (C) Upper panel represents in vitro kinase assays showing the dose-dependent effect of 2,4,6-THBA on CDK2 activity. Lower panel shows coomassie stained H1 histones. (D) Quantification of the blot in (C). (E) Upper panel represents the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA and 2,4,6-THBA on CDK4 activity. Lower panel shows coomassie stained retinoblastoma protein. (F) Quantification of the blot in (E). (G) Upper panel represents the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA (0.5 mM) and 2,4,6-THBA on CDK6 enzyme activity. Lower panel shows coomassie stained retinoblastoma protein. (H) Quantification of the blot in (G). * P<0.05, ** P<0.01, † P<0.001, ‡ P<0.001.

Article Snippet: CDK1/cyclin B1 active enzyme from New England Biolabs (NEB) (Ipswich, MA, USA), CDK1/cyclin B1, CDK2/cyclin A2, CDK4/cyclin D1, CDK6/cyclin D1, Retinoblastoma (C-term) and kinase buffer were purchased from SignalChem (Richmond, BC, Canada).

Techniques: Activity Assay, In Vitro, Staining

Journal: International Journal of Oncology

Article Title: Salicylic acid metabolites and derivatives inhibit CDK activity: Novel insights into aspirin's chemopreventive effects against colorectal cancer

doi: 10.3892/ijo.2017.4167

Figure Lengend Snippet: A model depicting how aspirin may preferentially act on colonic tissue to protect against CRC. We suggest that unabsorbed aspirin/salicylic acid in the stomach and upper intestine is passed on to the colon, taken up by the colonic epithelial cells, and metabolized by CYP450s to produce 2,3-DHBA and 2,5-DHBAs. These hydrophilic DHBAs may not easily cross the basolateral membrane of the epithelial cells and within these cells, they may accumulate to pharmacologically relevant concentrations, leading to the inhibition of CDK1 and CDK6, and exert anticancer effects. Colonic epithelial cells may also get exposed to salicylic acid metabolites generated by the gut microflora, and uptake of these HBAs by cells may also cause CDK inhibition. This additional layer of protection against tumor development may be unique to the colonic tissue allowing aspirin as a more effective drug against CRC. (?), Aspirin acetylates CDK1; however, how this modification affects its function is not clear at this stage.

Article Snippet: CDK1/cyclin B1 active enzyme from New England Biolabs (NEB) (Ipswich, MA, USA), CDK1/cyclin B1, CDK2/cyclin A2, CDK4/cyclin D1, CDK6/cyclin D1, Retinoblastoma (C-term) and kinase buffer were purchased from SignalChem (Richmond, BC, Canada).

Techniques: Inhibition, Generated, Modification

Journal: iScience

Article Title: Two birds with one stone: An antibiotic hit blocking Staphylococcus aureus heme uptake with serendipitous hemoglobin left-shifting activity

doi: 10.1016/j.isci.2026.115625

Figure Lengend Snippet: Structural comparison between Hb bound to C35 and Hb bound to INN298 (A) Chemical structure of the compound INN298. (B) Chemical structure of the compound C35. (C) Molecular surface of Hb bound to INN298 and its close-up on the INN298-binding site. (D) Molecular surface of Hb bound to C35 and its close-up on C35 binding site (PDB: 28OD ). INN298 and C35 are reported as cyan and magenta sticks, respectively. (E) Different tetrameric Hb structures superimposed on the α1 and β1 chains: HbCO (R state, PDB: 2DN3 , gray ribbons), Hb bound to INN298 (R2 state, PDB: 3IC0 , cyan ribbons), and Hb bound to C35 (R2 state, PDB: 28OD , magenta ribbons). Heme molecules are represented as sticks. The RMSD values estimated for Hb bound to INN298 or to C35 considering R-state Hb as a reference are 4.46 Å and 4.39 Å, respectively. The RMSD value between INN298- and C35-bound Hbs is 0.54 Å.

Article Snippet: C35-bound human hemoglobin structure , Protein DataBank , PDB: 28OD.

Techniques: Comparison, Binding Assay